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gclm  (Novus Biologicals)
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
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gclm antibody nbmp1-33405  (Novus Biologicals)
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
Gclm Antibody Nbmp1 33405, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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antibodies gclm nbmp1-33405  (Novus Biologicals)
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
Antibodies Gclm Nbmp1 33405, supplied by Novus Biologicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
Dhfr Deficient Cho Dg44, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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mass flow controller 33405  (Alicat Scientific)
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
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cho gd44  (ATCC)
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<t>(A)</t> <t>NRF2,</t> <t>GCLM,</t> and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.
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Image Search Results


(A) NRF2, GCLM, and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.

Journal: Cell reports

Article Title: A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity

doi: 10.1016/j.celrep.2019.01.043

Figure Lengend Snippet: (A) NRF2, GCLM, and MRP1 protein levels in six cancer cell lines of diverse tissue origin. (B) Pearson correlation of NRF2 protein levels to GCLM and MRP1 protein levels in the cell lines from (A). Protein expression was determined from three independent experiments and the levels of each protein normalized to those observed in HAP1 cells, which was set equal to 1. (C) Pearson correlation of NRF2, GCLM, and MRP1 protein expression to intracellular glutathione levels in these cell lines. (D) Immunoblotting of lysates from A549 N Control and MRP1 KO1/2 cells using antibodies against MRP1, NRF2, and α-tubulin. (E) The timing of population cell death onset (D O ) determined in A549 N Control and MRP1 KO1/2 cells using STACK. (F) Intracellular glutathione levels determined using Ellman’s reagent. BLD, below the limit of detection. (G) Extracellular GSH release determined by stable isotope tracing and mass spectrometry. (H) Pearson correlation between NRF2, GCLM, or MRP1 protein levels and erastin2 potency in the six cell lines from (A). (I) Pearson correlation between erastin2 potency and intracellular glutathione levels in the six cell lines from (A). Data are means ± 95% confidence intervals (B, C, E, H, and I) or means ± SDs (F and G) from three independent experiments. Data in (F) and (G) were analyzed using one-way ANOVA, with *p < 0.05; ns, not significant.

Article Snippet: Membranes were probed with primary antibodies against MRP1 (Cat#ab3368, Abcam, 1:500 dilution), KEAP1 (Cat# sc-365626, Santa Cruz, 1:500 dilution), NRF2 (Cat# ab137550, Abcam, 1:1000 dilution), GCLM (Cat# NBP1-33405, Novus Biologicals), and alpha-tubulin (Cat# MS581P1, Fisher Scientific) in Odyssey Buffer (Cat# 927-40100, LI-COR Biotechnology, Lincoln, NE) (60 rpm, 4°C, overnight).

Techniques: Expressing, Western Blot, Control, Mass Spectrometry

KEY RESOURCES TABLE

Journal: Cell reports

Article Title: A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity

doi: 10.1016/j.celrep.2019.01.043

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Membranes were probed with primary antibodies against MRP1 (Cat#ab3368, Abcam, 1:500 dilution), KEAP1 (Cat# sc-365626, Santa Cruz, 1:500 dilution), NRF2 (Cat# ab137550, Abcam, 1:1000 dilution), GCLM (Cat# NBP1-33405, Novus Biologicals), and alpha-tubulin (Cat# MS581P1, Fisher Scientific) in Odyssey Buffer (Cat# 927-40100, LI-COR Biotechnology, Lincoln, NE) (60 rpm, 4°C, overnight).

Techniques: Virus, Recombinant, In Vitro, Transfection, DNA Extraction, RNA Extraction, Reverse Transcription, Purification, SYBR Green Assay, Bicinchoninic Acid Protein Assay, Bradford Assay, Control, Software